Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CAAT/enhancer binding protein is able to bind to ATF/CRE elements.

O Bakker1, M G Parker

  • 1Molecular Endocrinology Laboratory, Imperial Cancer Research Fund, London, UK.

Nucleic Acids Research
|March 25, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uterine Hemorrhage.

The North-Western medical and surgical journal·2023
Same author

Educational level and use of osteoporosis drugs in elderly men and women: a Swedish nationwide register-based study.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2012
Same author

Cognitive impairments in adult mice with constitutive inactivation of RIP140 gene expression.

Genes, brain, and behavior·2011
Same author

Kidney allograft biopsy: timing to complications.

Clinical nephrology·2010
Same author

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data.

Nucleic acids research·2009
Same author

IL-1B -31T>C promoter polymorphism is associated with gastric stump cancer but not with early onset or conventional gastric cancers.

Virchows Archiv : an international journal of pathology·2008

The transcription factor C/EBP binds to ATF/CRE sites, similar to enhancer core elements. This binding enables C/EBP to direct gene transcription, with mutations affecting binding also impacting transcriptional activity.

Area of Science:

  • Molecular Biology
  • Transcription Factor Regulation
  • Gene Expression

Background:

  • CCAAT/enhancer-binding proteins (C/EBP) are transcription factors known to bind CAAT-box and viral enhancer elements.
  • The binding capabilities and transcriptional roles of C/EBP at specific DNA sequences are areas of ongoing research.

Purpose of the Study:

  • To investigate the binding affinity of C/EBP to ATF/CRE sites.
  • To determine if C/EBP can functionally regulate transcription via ATF/CRE sites.
  • To elucidate the mechanism by which C/EBP interacts with ATF/CRE sites.

Main Methods:

  • Electrophoretic mobility shift assays (band-shift assays) to assess DNA-protein binding.
  • Methylation interference assays to identify key DNA-binding contacts.

Related Experiment Videos

  • DNase I footprinting to map protein binding sites.
  • Transient transfection assays to evaluate transcriptional activity.

    • Main Results:

    • C/EBP exhibits high-affinity binding to ATF/CRE sites.
    • Homology between ATF/CRE sites and enhancer core elements may facilitate C/EBP binding.
    • C/EBP successfully directs transcription from ATF/CRE sites in reporter gene assays.
    • Impairment of DNA binding at mutated ATF sites correlates with reduced C/EBP-mediated transcriptional activation.

    Conclusions:

    • C/EBP possesses a broader DNA-binding specificity than previously recognized, including ATF/CRE sites.
    • The interaction of C/EBP with ATF/CRE sites is functionally significant for gene transcription.
    • Structural similarities between binding sites underpin C/EBP's versatile DNA recognition and regulatory functions.