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Updated: Jun 14, 2026

A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)
Published on: April 30, 2020
Front-loading sputum microscopy services: an opportunity to optimise smear-based case detection of tuberculosis in
Andy Ramsay1, Mohammed Ahmed Yassin, Alexis Cambanis
1Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK.
Abstract:
Setting. Ethiopia, Nepal, Nigeria, and Yemen. Objective. To reduce the time to complete sputum microscopy. Design. Cross-sectional surveys enrolling 923 patients with chronic cough in the 4 countries and using similar protocols. Spot-morning-spot sputum specimens were collected. An additional sputum specimen (Xspot) was collected one hour after the first, and the yields of the first two or the three specimens collected as spot-morning-spot or spot-Xspot-morning were compared. Results. 216 patients had >/= one positive smear. 210 (97%) were identified by the spot-morning-spot, and 210 (97%) were identified by the spot-Xspot-morning specimens, with 203 and 200 identified by the first 2 specimens of each approach, respectively. Neither difference was significant. Conclusions. The time to complete smear microscopy could be reduced.
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