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Published on: November 18, 2011
Cryopreservation of Thymus moroderi by droplet vitrification
A Marco-Medina1, J Luis Casas, R Swennen
1Institute of Biodiversity, University of Alicant, Alicante, Spain.
Cryo Letters
|March 24, 2010
Abstract:
Thymus moroderi Pau ex Martinez (Labiatae) was successfully cryopreserved using the droplet vitrification method. After 20 min in loading solution at room temperature, shoot tips were dehydrated with PVS2 at 0 degree C for 30 min and immersed into LN. For thawing, shoot-tips were transferred into recovery solution for 15 min. A test of different recovery media revealed that the best results were obtained when the medium was supplement with 0.275 micromolar BA.
