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Overexpression, purification and crystallization of BamHI endonuclease
W E Jack1, L Greenough, L F Dorner
1New England Biolabs, Inc., Beverly, MA 01915.
Nucleic Acids Research
|April 25, 1991
Abstract:
The type II restriction endonuclease BamHI has been expressed in E. coli, producing 100-fold more enzyme than the wild type Bacillus amyloliquefaciens H strain. This high yield has facilitated purification to homogeneity of large amounts of the enzyme, along with its crystallization in a form which diffracts to at least 1.9 A in X-ray analysis.