A discussion on the process of defining 2-D separation selectivity
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Updated: Jun 14, 2026

Spatial Separation of Molecular Conformers and Clusters
Published on: January 9, 2014
Paul G Stevenson1, Mariam Mnatsakanyan, Andrew R Francis
1Australian Centre for Research on Separation Science and the Nanoscale Organisation and Dynamics Group, and School of Natural Sciences, University of Western Sydney, Parramatta, NSW, Australia.
Abstract:
In this manuscript, we investigate the importance that must be placed on the selection of standard compounds when undertaking studies to optimize the performance of 2-D-HPLC separations. A geometric approach to factor analysis and a measure of peak density across the separation space were applied to assess localized measures of component distributions within the 2-D separation plane. The results of this analysis of data showed that the measure of separation quality varied markedly, depending on the elution zone for which the test was undertaken. The study concluded that if standards cannot be obtained that adequately describe the entire sample matrix, the sample itself should be used, and also, the separation should be optimized for regions of interest, not necessarily the separation as a whole.
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