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Why no simple relationship between thyroid peroxidase activity-inhibiting immunoglobulins and thyroid function in
Y Okamoto1, N Hamada, T Fujisawa
1Second Department of Internal Medicine, Osaka City University Medical School, Japan.
Acta Endocrinologica
|April 1, 1991
Summary
Thyroid peroxidase activity-inhibiting immunoglobulins can hinder thyroid function, but their relationship isn't straightforward. These antibodies may struggle to reach their target antigen, explaining the complex connection to thyroid function.
Area of Science:
- Endocrinology
- Immunology
- Pathology
Background:
- Anti-thyroid peroxidase antibodies can inhibit thyroid peroxidase activity in vitro.
- The clinical impact of these inhibitory antibodies on thyroid function is complex and not fully understood.
Purpose of the Study:
- To investigate the reasons behind the complex relationship between thyroid peroxidase activity-inhibiting immunoglobulins and thyroid function.
- To determine the accessibility of thyroid peroxidase to inhibitory antibodies in different thyroid tissue preparations.
Main Methods:
- Immunofluorescence staining and peroxidase-antiperoxidase methods were used to detect immunoglobulin G deposits.
- Enzyme histochemistry was employed to assess endogenous thyroid peroxidase activity.
- Thyroid sections (cryostat and paraffin-embedded) and organ-cultured thyroid slices were utilized.
Main Results:
- Incubation with inhibitory immunoglobulins inhibited thyroid peroxidase activity in cryostat sections, showing apical and intracellular immunoglobulin G staining.
- No immunoglobulin G deposits were observed on the apical border in paraffin-embedded sections from Hashimoto's and Graves' patients.
- Thyroid peroxidase activity remained uninhibited in organ-cultured thyroid slices incubated with inhibitory immunoglobulins.
Conclusions:
- Thyroid peroxidase activity-inhibiting immunoglobulins may have limited access to their antigen in situ.
- Difficulty in antigen accessibility is a significant factor contributing to the observed complex relationship between these immunoglobulins and thyroid function.