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[HLA B27 phenotyping using flow cytometry].

R Kravtzoff, S Canepa, M D Boulanger

    Revue Francaise De Transfusion Et D'Hemobiologie : Bulletin De La Societe Nationale De Transfusion Sanguine
    |March 1, 1991
    PubMed
    Summary
    This summary is machine-generated.

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    Flow cytometry for HLA B27 antigen typing is a rapid screening tool. While it avoids false negatives, positive results require confirmation due to potential cross-reactions with the B7 group.

    Area of Science:

    • Immunogenetics
    • Cellular immunology
    • Biotechnology

    Context:

    • Human Leukocyte Antigen (HLA) B27 typing is crucial for diagnosing certain autoimmune diseases.
    • Traditional microlymphocytotoxicity testing is time-consuming and requires specialized expertise.
    • Advancements in flow cytometry offer potential for faster and more accessible HLA typing.

    Purpose:

    • To evaluate the efficacy of flow cytometry using a FITC monoclonal anti-HLA B27 antibody for antigen typing.
    • To compare flow cytometry results with the established microlymphocytotoxicity test.
    • To determine the reliability and limitations of flow cytometry as a screening method for HLA B27.

    Summary:

    • Flow cytometry analysis of 106 patients for HLA B27 antigens demonstrated no false-negative results.

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  • However, cross-reactivity of the monoclonal antibody with the CREG B7 group can lead to false-positive results.
  • The study suggests flow cytometry is a valuable rapid screening technique for HLA B27, with negative results being reliable.

    • Impact:

    • Flow cytometry provides a rapid and efficient screening method for HLA B27 antigen detection.
    • This technique can expedite the diagnostic process for HLA B27-associated conditions.
    • Confirmation of positive results is necessary, highlighting the complementary role of traditional methods.