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The synthesis of phosphoenolpyruvate carboxylase in imbibing sorghum seeds
E Khayat1, E B Dumbroff, B R Glick
1Department of Biology, University of Waterloo, Ont., Canada.
Abstract:
When sorghum seeds were imbibed either in the light or in the dark, the presence of newly synthesized phosphoenolpyruvate carboxylase (PEPC) could be detected immunologically after approximately 6 h. In addition, both PEPC mRNA and enzyme activity were detected in extracts of dry seeds prior to inhibition. By contrast, ribulose-1,5-bisphosphate carboxylase mRNA, protein, and activity, as well as chlorophyll, were not detected even after 24 h of inhibition. These observations suggest that the nonphotosynthetic form of PEPC is synthesized during seed development and may play an important role in the germinative process.
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