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Lowered superoxide dismutase in highly metastatic B16 melanoma cells
J K Kwee1, E Mitidieri, O R Affonso
1Centro de Pesquisa Básica, Instituto Nacional de Câncer Praça Cruz Vermelha, Rio de Janeiro, Brasil.
Abstract:
Superoxide dismutases (SOD), which are enzymes scavenging the superoxide radical, were studied in two variant lines of the B16 melanoma: B16F1 with low metastatic potential and B16F10 with high metastatic potential. SOD activity was measured by a method utilizing reduction in the chemiluminescence of luminol. Using cell free extracts it was shown that the highly metastatic B16F10 cell line has a SOD activity lower (20.70 +/- 3.07) units/mg protein, n = 8, than that of the less metastatic B16F1 cell line (81.38 +/- 6.78) units/mg protein, n = 8. Acrylamide gel electrophoresis suggested that Mn-SOD activity is higher in B16F1 cells.