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A new incompatibility group plasmid in Staphylococcus aureus.

E E Udo¹, W B Grubb

¹School of Medical Technology, Curtin University of Technology, Perth, WA, Australia.

FEMS Microbiology Letters

|February 1, 1991

Summary

New plasmid incompatibility groups were identified in Staphylococcus aureus. The conjugative plasmids pWBG637, pWBG636, and pWBG642 define a novel Incompatibility group 15, expanding our understanding of plasmid biology.

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Area of Science:

Microbiology
Genetics
Molecular Biology

Background:

Plasmids are crucial for bacterial adaptation and antibiotic resistance.
Understanding plasmid incompatibility is essential for managing their spread in bacterial populations.
Staphylococcus aureus is a significant human pathogen where plasmid dynamics are actively studied.

Purpose of the Study:

To determine the incompatibility of conjugative plasmids pWBG637, pWBG636, and pWBG642 in Staphylococcus aureus.
To establish whether these plasmids belong to previously defined incompatibility groups.
To define a new incompatibility group if the tested plasmids do not match existing ones.

Main Methods:

Conjugative and non-conjugative plasmids were introduced into Staphylococcus aureus.

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Plasmid incompatibility was assessed by attempting to maintain multiple plasmids within the same bacterial cell.

The tested plasmids (pWBG637, pWBG636, pWBG642) were evaluated against existing incompatibility groups.

Main Results:

The conjugative plasmids pWBG637, pWBG636, and pWBG642 demonstrated compatibility with other conjugative plasmids.
These plasmids were also compatible with plasmids from the 14 established incompatibility groups.
No incompatibility was observed with any of the existing groups.

Conclusions:

The tested plasmids, pWBG637, pWBG636, and pWBG642, represent a distinct set of replicons.
These findings establish a new Incompatibility group, designated as Incompatibility group 15.
This discovery contributes to the classification of plasmids in Staphylococcus aureus and aids in tracking plasmid transmission.