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Delta-like haemolysin produced by Staphylococcus lugdunensis.

F Vandenesch¹, M J Storrs, F Poitevin-Later

¹Centre National de Référence des Staphylocoques, Lyon, France.

FEMS Microbiology Letters

|February 1, 1991

Summary

Staphylococcus lugdunensis clinical isolates often produce a thermostable hemolysin. Genetic analysis reveals these strains possess a delta-like toxin homologous to Staphylococcus aureus delta toxin.

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Area of Science:

Microbiology
Bacterial Pathogenesis
Molecular Biology

Background:

Staphylococcus lugdunensis is a coagulase-negative staphylococcal species.
Some S. lugdunensis strains exhibit hemolytic activity, similar to Staphylococcus aureus.
The genetic basis for S. lugdunensis hemolysin production is not fully understood.

Purpose of the Study:

To investigate the hemolytic activity of clinical isolates of Staphylococcus lugdunensis.
To determine the presence of DNA sequences homologous to Staphylococcus aureus toxin genes in S. lugdunensis.

Main Methods:

Testing 30 clinical isolates of S. lugdunensis for hemolysin production.
Assessing hemolysin activity against rabbit erythrocytes.
Performing DNA-DNA hybridization using probes for S. aureus alpha, beta, delta, and gamma toxin genes, and coagulase gene.

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Main Results:

Twenty-six of 30 S. lugdunensis strains produced thermostable hemolysin.
DNA hybridization revealed homology with the S. aureus delta toxin gene probe in 28 strains.
Six strains showed homology to the delta probe despite lacking detectable hemolysin expression.

Conclusions:

Staphylococcus lugdunensis frequently produces a thermostable hemolysin.
S. lugdunensis possesses a delta-like toxin gene that shares homology with the Staphylococcus aureus delta toxin gene.
The presence of the delta-like toxin gene may not always correlate with observable hemolysin activity.