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Related Experiment Videos

Proteoglycan molecules in keratoconus corneas.

S Sawaguchi1, B Y Yue, I Chang

  • 1Department of Ophthalmology, Lions of Illinois Eye Research Institute, University of Illinois, Chicago 60612.

Investigative Ophthalmology & Visual Science
|May 1, 1991
PubMed
Summary

Keratoconus corneas show altered proteoglycan composition, with increased dermatan sulfate proteoglycans and decreased keratan sulfate proteoglycans. These changes, particularly in scarred areas, suggest they may be secondary to corneal scarring.

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Area of Science:

  • Ophthalmology
  • Biochemistry
  • Histology

Background:

  • Keratoconus is a progressive thinning of the cornea.
  • Proteoglycans play a crucial role in maintaining corneal structure and hydration.
  • Alterations in proteoglycan composition may contribute to keratoconus pathogenesis.

Purpose of the Study:

  • To investigate the alterations in proteoglycan molecules within keratoconus corneas.
  • To compare proteoglycan profiles in keratoconus corneas with normal human corneas.
  • To explore the potential relationship between proteoglycan changes and corneal scarring in keratoconus.

Main Methods:

  • Immunohistochemical analysis using monoclonal antibodies (9-A-2 and J-19).
  • Electron microscopy with cuprolinic blue staining.

Related Experiment Videos

  • Uronic acid analyses.

    • Main Results:

    • Increased immunoreactivity for dermatan sulfate proteoglycan epitopes (antibody 9-A-2) in keratoconus stroma.
    • Decreased immunoreactivity for sulfated keratan sulfate proteoglycan epitopes (antibody J-19) in keratoconus.
    • Electron microscopy revealed abnormally thick, chondroitinase ABC-sensitive dermatan sulfate proteoglycan filaments and reduced keratan sulfate proteoglycan filaments in keratoconus, especially in scarred regions.

    Conclusions:

    • Keratoconus corneas exhibit significant alterations in both dermatan sulfate and keratan sulfate proteoglycans.
    • The observed proteoglycan abnormalities, particularly the accumulation of dermatan sulfate proteoglycan filaments, are more pronounced in scarred areas.
    • These findings suggest that proteoglycan alterations in keratoconus may be, at least partly, a secondary consequence of corneal scarring.