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Updated: Jun 14, 2026

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
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Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

Published on: May 25, 2019

[Rapid method to extract high-quality RNA from activated sludge].

Min Jin1, Zu-Guo Zhao, Zhi-Gang Qiu

  • 1Department of Environmental Health and Engineering, Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China. jinminzh@126.com

Huan Jing Ke Xue= Huanjing Kexue
|March 25, 2010
PubMed
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A new RNA isolation method for activated sludge offers high yield, purity, and integrity. This cost-effective technique enables accurate microbial community analysis using RT-PCR and T-RFLP.

Area of Science:

  • Environmental microbiology
  • Molecular biology techniques

Background:

  • Activated sludge is crucial for wastewater treatment.
  • Understanding microbial communities in activated sludge is vital for process optimization.
  • Efficient RNA extraction is necessary for molecular analysis of microbial communities.

Purpose of the Study:

  • To establish an effective and rapid RNA isolation method for activated sludge.
  • To compare five different RNA extraction methods.
  • To evaluate the suitability of extracted RNA for downstream molecular analyses.

Main Methods:

  • Comparison of five RNA isolation methods for activated sludge.
  • Utilized TRIzol reagent, lysozyme, chloroform, isopropanol, DNase I, and RNA purification columns.
  • Assessed RNA yield, purity, integrity, and RT-PCR amplification of 16S rRNA and amoA genes.

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Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
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Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Published on: June 11, 2016

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Last Updated: Jun 14, 2026

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
08:53

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

Published on: May 25, 2019

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
10:51

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

Published on: October 31, 2014

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
11:37

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Published on: June 11, 2016

  • Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis was performed.

    • Main Results:

    • The optimized method using TRIzol and an RNA purification kit yielded high-quality RNA (high quantity, purity, integrity, low degradability).
    • Extracted RNA allowed successful RT-PCR amplification of 16S rRNA and amoA genes.
    • The method demonstrated cost-effectiveness and high efficiency compared to others.
    • T-RFLP analysis showed that RNA extraction methods impact microbial community composition and abundance.

    Conclusions:

    • A rapid, effective, and cost-efficient RNA isolation method for activated sludge was developed.
    • This method provides high-quality RNA suitable for molecular studies.
    • The established method has potential for monitoring microbial dynamics, metabolism, and community structure in activated sludge.