Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Introduction to "Endocytosis and cellular delivery".

RSC chemical biology·2026
Same author

Interactions of POPC Liposomes with Graphene Investigated by Quartz Crystal Microbalance and All-Atom Molecular Dynamics Simulations.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Near-Quantitative Formation of Imines in Water with Allosteric Control.

Journal of the American Chemical Society·2026
Same author

Identification and Characterization of an In Silico Designed Membrane-Active Peptide with Antiviral Properties.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

CD44-targeted lipid nanoparticles for enhanced CRISPR/Cas9 delivery in cancer gene editing.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

A general logic-gating framework for CAR-T and nanocarrier cancer therapies: A cross-platform comparative analysis of logic architectures and their molecular implementation strategies.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same journal

Multicomponent Micelles with Boosted Stability of Iminoboronates.

Biomacromolecules·2026
Same journal

Stiffening and Toughening Protein Hydrogels by Tuning Electrostatic Interactions.

Biomacromolecules·2026
Same journal

<i>In Situ</i> Bulk and Interfacial Interlocking-Induced Highly Dynamically Entangled Hydrogel of Myocardium-Matching Mechanics, Electrophysiological Functions, and Robust Tissue Adhesion for Cardiac Repair.

Biomacromolecules·2026
Same journal

Eutectogel Electrodes with Self-powered Capability for Flexible Electrophysiological Sensor.

Biomacromolecules·2026
Same journal

Self-Reporting Supramolecular Coacervates Driven by Liquid-Liquid Phase Separation Enable Systemic Translocation and Photodynamic Bioprotection.

Biomacromolecules·2026
Same journal

Self-Assembed G-Quadruplex Nanowires for Energy Transfer over Micrometers.

Biomacromolecules·2026
See all related articles

Related Experiment Video

Updated: Jun 14, 2026

Forming Giant-sized Polymersomes Using Gel-assisted Rehydration
08:45

Forming Giant-sized Polymersomes Using Gel-assisted Rehydration

Published on: May 26, 2016

Detergent-aided polymersome preparation.

Hana Robson Marsden1, Christophe Barnier Quer, Eduardo Y Sanchez

  • 1Department of Soft Matter Chemistry, Leiden Institute of Chemistry and Division of Drug Delivery Technology, Leiden/Amsterdam Center for Drug Research, Leiden University, P.O. Box 9502, 2300RA Leiden, The Netherlands.

Biomacromolecules
|March 25, 2010
PubMed
Summary
This summary is machine-generated.

A new detergent-aided method creates polymersomes, a type of polymeric vesicle, avoiding harsh organic solvents or sonication. This technique is ideal for encapsulating delicate biomacromolecules, preserving their activity.

More Related Videos

Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure
06:01

Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure

Published on: April 21, 2021

Facile Synthesis of Worm-like Micelles by Visible Light Mediated Dispersion Polymerization Using Photoredox Catalyst
07:39

Facile Synthesis of Worm-like Micelles by Visible Light Mediated Dispersion Polymerization Using Photoredox Catalyst

Published on: June 8, 2016

Related Experiment Videos

Last Updated: Jun 14, 2026

Forming Giant-sized Polymersomes Using Gel-assisted Rehydration
08:45

Forming Giant-sized Polymersomes Using Gel-assisted Rehydration

Published on: May 26, 2016

Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure
06:01

Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure

Published on: April 21, 2021

Facile Synthesis of Worm-like Micelles by Visible Light Mediated Dispersion Polymerization Using Photoredox Catalyst
07:39

Facile Synthesis of Worm-like Micelles by Visible Light Mediated Dispersion Polymerization Using Photoredox Catalyst

Published on: June 8, 2016

Area of Science:

  • Polymer Chemistry
  • Materials Science
  • Nanotechnology

Background:

  • Traditional methods for creating polymeric vesicles (polymersomes) often rely on organic cosolvents or sonication.
  • These methods can be detrimental to the integrity and activity of encapsulated sensitive biomolecules.

Purpose of the Study:

  • To introduce a novel, milder method for synthesizing polymersomes using a detergent-aided approach.
  • To demonstrate the versatility of this method with different block copolymers.

Main Methods:

  • Utilized a detergent-aided self-assembly process starting with block copolymer solubilization in detergent micelles.
  • Reduced detergent concentration via dilution to induce the transformation from micelles to polymersomes.
  • Employed dynamic light scattering and transmission electron microscopy for characterization.

Main Results:

  • Successfully produced polymersomes from a rod-rod block copolymer (PBLG-E) with average diameters around 300 nm and discs around 100 nm.
  • Demonstrated the method's applicability to a flexible block copolymer (polybutadiene-b-poly(ethylene glycol)).
  • Confirmed the formation and morphology of the polymersomes.

Conclusions:

  • The detergent-aided method offers a gentle and effective alternative for polymersome synthesis.
  • This technique is particularly advantageous for encapsulating delicate biomacromolecules, preserving their biological activity.
  • The method's adaptability allows for the creation of polymersomes with diverse properties.