Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Amperometry: Overview

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...

Gas Chromatography: Types of Detectors-II

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nitric oxide-releasing balloon inflation fluid for Foley catheters to prevent catheter-associated urinary tract infections.

Microbiology spectrum·2026

Same author

Nitric Oxide-Releasing Surfaces Reduce Thrombosis in Venovenous Extracorporeal Life Support: A 5 day Long Ovine Study.

ASAIO journal (American Society for Artificial Internal Organs : 1992)·2026

Same author

Photolysis of solid-state S-nitrosoglutathione: Mechanism and kinetics of nitric oxide release.

Nitric oxide : biology and chemistry·2026

Same author

Cryopreservable dopaminergic progenitors derived from human iPSCs with accelerated loss of pluripotency and early functional restoration in Parkinsonian rats.

NPJ Parkinson's disease·2026

Same author

HYPER-Net: Physics-Conditioned Self-Supervised Reconstruction for Fourier Light-Field Microscopy.

bioRxiv : the preprint server for biology·2026

Same author

Liposomal composition tuning improves intranasal brain drug delivery: Role of cholesterol and formulation rheology.

International journal of biological macromolecules·2026

Search research articles

Related Experiment Video

Updated: Jun 14, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Patterned electrode-based amperometric gas sensor for direct nitric oxide detection within microfluidic devices.

Wansik Cha¹, Yi-Chung Tung, Mark E Meyerhoff

¹Department of Biomedical Engineering and Macromolecular Science & Engineering Center, University of Michigan, 2200 Bonisteel Boulevard, Ann Arbor, Michigan 48109-2099, USA.

Analytical Chemistry

|March 25, 2010

Summary

This summary is machine-generated.

This study presents a thin, microchannel-embedded amperometric sensor for real-time nitric oxide (NO) detection in cell cultures. The novel sensor design offers high sensitivity and selectivity for NO monitoring in biological samples.

More Related Videos

NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose

NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose

Published on: December 30, 2025

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Related Experiment Videos

Last Updated: Jun 14, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose

NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose

Published on: December 30, 2025

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Area of Science:

Biosensors
Analytical Chemistry
Materials Science

Background:

Nitric oxide (NO) is a crucial signaling molecule in biological systems.
Accurate real-time detection of NO in cellular environments is challenging.
Existing NO sensors often lack selectivity and sensitivity for biological applications.

Purpose of the Study:

To develop a thin, microchannel-embedded amperometric sensor for direct, real-time NO detection.
To integrate the sensor into a microfluidic device for cell culture monitoring.
To achieve high sensitivity and selectivity for NO in complex biological media.

Main Methods:

Fabrication of a thin planar sensor using patterned gold/indium-tin oxide on a porous polymer membrane (pAu/ITO).
Electrochemical deposition of an Au-hexacyanoferrate layer for NO catalysis and current stabilization.
Integration of a gas-permeable membrane for enhanced selectivity against interfering agents.
Utilizing a microfluidic device for cell culture and sensor integration.

Main Results:

The sensor achieved a detection limit of approximately 1 nM in stirred buffer and <10 nM in flowing buffer.
The sensor demonstrated excellent selectivity for NO over common interfering agents like nitrite and ascorbate.
The integrated sensor successfully monitored NO generation from stimulated macrophages in microchannels.

Conclusions:

The developed thin amperometric sensor is suitable for microchannel embedding and real-time NO detection in cell cultures.
The sensor design provides high sensitivity, selectivity, and stability for NO monitoring in biological fluids.
This technology enables advanced studies of cellular NO production within microfluidic systems.