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Updated: Jun 14, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Diversity and phylogeny of mitochondrial DNA isolated from mithun Bos frontalis located in Bhutan
T Dorji1, H Mannen, T Namikawa
1Department of Livestock, Ministry of Agriculture, Royal Government of Bhutan, Thimphu, Bhutan.
Abstract:
We sequenced the 16S rRNA gene in mitochondrial DNA to characterize mithun located in Bhutan and to increase our understanding of its origin. We compared mithun with yak, European cattle, Bhutanese zebu and Indian zebu. Sequencing revealed low nucleotide diversity within the mithun population and their phylogenetic proximity to gaur. A close relationship between Bhutanese mithun and gaur was confirmed by an additional comparison with wild gaur specimens from three locations in Bhutan. Direct domestication of mithun from gaur was supported, while maternal contribution from the cattle lineage during domestication was not supported.
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