Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Human Virome

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

Viruses of Archaea

Viruses of Archaea

Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...

Evolution of Microbial Genome

Evolution of Microbial Genome

Microbial genome evolution is a highly dynamic process shaped by continual gene gain and loss across species and strains. This genomic flexibility allows microorganisms to adapt rapidly to environmental pressures and interactions with other organisms. Central to understanding this diversity is the distinction between the core and pan genomes.The core genome comprises the genes shared by all sampled strains of a species, representing essential functions needed for fundamental cellular processes.

Bacteriophages of the Human Virome

Bacteriophages of the Human Virome

Bacteriophages are found throughout the human body. They may even outnumber eukaryotic viruses, forming an important and dynamic component of the human virome. Indeed, phages represent the most abundant viral entities, with densities in the gut reaching up to 10⁹ particles per gram of fecal matter, and many belonging to orders such as Caudovirales and Microviridae, while a substantial proportion remains unclassified as viral “dark matter.”Lysogeny and Genetic ExchangeIn the gut, bacteriophages...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comprehensive analysis of the chloroplast genome structure and phylogeny of <i>Glochidion puberum</i> (L.) Hutch.

Mitochondrial DNA. Part B, Resources·2026

Same author

A near-complete, haplotype-resolved telomere-to-telomere genome assembly of Cannabis sativa reveals complex higher-order repetitive structures.

Plant communications·2026

Same author

Reusable Instrumentation for Arthroscopic Rotator Cuff Repair May Not Impact Clinical Outcomes.

Journal of the American Academy of Orthopaedic Surgeons. Global research & reviews·2026

Same author

The complete chloroplast genome of <i>Calophyllum membranaceum</i> Gander & Champ 1849 (Calophyllaceae, Malpighiales) and its phylogenetic analysis.

Mitochondrial DNA. Part B, Resources·2026

Same author

The complete chloroplast genome and phylogenetic analysis of <i>Schisandra henryi</i> C. B. Clarke.

Mitochondrial DNA. Part B, Resources·2026

Same author

A manganese metabolism-related gene signature for prognosis prediction and immune microenvironment description of glioblastoma.

Discover oncology·2026

Search research articles

Related Experiment Video

Updated: Jun 14, 2026

Environmental Sampling of Photosynthetic Microbes and Their Viruses: From Field to Lab

Environmental Sampling of Photosynthetic Microbes and Their Viruses: From Field to Lab

Published on: July 3, 2025

Ecogenomics: using massively parallel pyrosequencing to understand virus ecology.

Marilyn J Roossinck¹, Prasenjit Saha, Graham B Wiley

¹The Samuel Roberts Noble Foundation, Ardmore, OK 73402, USA. mroossinck@noble.org

Molecular Ecology

|March 25, 2010

Summary

This summary is machine-generated.

Researchers developed a new method to identify plant viruses and their hosts. This technique analyzes double-stranded RNA from individual plants, enabling the discovery of thousands of new viruses linked to their specific hosts.

More Related Videos

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

Related Experiment Videos

Last Updated: Jun 14, 2026

Environmental Sampling of Photosynthetic Microbes and Their Viruses: From Field to Lab

Environmental Sampling of Photosynthetic Microbes and Their Viruses: From Field to Lab

Published on: July 3, 2025

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

Area of Science:

Virology
Plant Pathology
Genomics

Background:

Metagenomic studies have analyzed environmental samples for viruses.
Previous studies could not link individual viruses to their specific hosts.
Identifying plant viruses and their hosts is crucial for understanding plant health and biodiversity.

Purpose of the Study:

To develop a novel strategy for isolating and identifying plant viruses and their hosts.
To overcome the limitations of previous metagenomic approaches in linking viruses to hosts.
To enable large-scale analysis of plant virus biodiversity.

Main Methods:

Isolation of double-stranded RNA (dsRNA), a marker of RNA virus infection, from individual plant samples.
Conversion of dsRNA to complementary DNA (cDNA) with unique four-nucleotide tags at each end.
Pooling of tagged cDNA samples (up to 96 samples per pool) for analysis.
Massively parallel sequencing of pooled samples using Roche 454 pyrosequencing to analyze up to 384 samples per picotiter plate.

Main Results:

Successful analysis of thousands of individual plant samples.
Discovery of several thousand novel plant viruses.
Direct linkage of newly discovered viruses to their specific plant hosts.
Detailed description and analysis of eight sample pools.

Conclusions:

The developed method effectively links newly discovered plant viruses to their hosts.
This technology significantly advances the understanding of plant virus biodiversity.
The method provides a powerful tool for large-scale plant virus discovery and host association studies.