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Updated: Jun 14, 2026
Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons
Published on: February 5, 2015
Detection and quantification of maitotoxin-like compounds using a neuroblastoma (Neuro-2a) cell based assay.
A Caillaud1, T Yasumoto, J Diogène
1IRTA, Sant Carles de la Ràpita, Ctra. Poble Nou s/n, 43540 Sant Carles de la Ràpita, Spain.
Abstract:
SK&F 96365 was used in a Neuroblastoma (Neuro-2a) cell based assay to determine the production of maitotoxin-like (MTX-like) compounds in two strains of Gambierdiscus spp. A 2.5 hour assay was effective for the detection of the MTX-induced toxic effects with a concentration that inhibited 50% cell viability (IC(50)) equivalent to 3.38 nM MTX. Evidence was found for the production of MTX-like compounds in both Gambierdiscus strains studied at concentrations of 404 and 36.7 nmoles MTX equivalence per 10(6) cells. The assay is proposed as an efficient approach to the detection and quantification of MTX-like compounds in Gambierdiscus spp.
