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Updated: Jun 14, 2026

The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers
Published on: January 22, 2013
Significant expression patterns of lewis X-related antigens as a prognostic predictor of low-stage renal cell
Minoru Kobayashi1, Tatsuo Morita
1Department of Urology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan. minoruk@jichi.ac.jp
Aim:
To examine expression profiles of LewisX(Le(x))-related antigens, which have a possible role in hematogenous metastatic spread in various malignancies, in renal cell cancer (RCC) tissue in order to evaluate their prognostic value for patients with low-stage RCC.
Patients And Methods:
Fifty-two patients with pT1-2N0M0 disease were evaluated for their expression patterns of Le(x)-related antigens using FH6 and CSLEX1 antibodies for sLe(x) and AG223 for 6-sulfo Le(x), immunohistochemically.
Results:
None of the expression levels of Le(x)-related antigens directed by the three antibodies related to the clinical outcomes of patients with low-stage RCC. However, combined use of FH6 and CSLEX1 antibodies enabled prognostic prediction, namely that patients with low intensity with FH6 and high intensity with CSLEX1 had a higher chance of disease progression and poor survival.
Conclusion:
Expression levels Le(x)-related antigens determined by combined use of FH6 and CSLEX1 antibodies could be of value as a prognostic indicator for patients with low-stage RCC.
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