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Related Experiment Videos

Antithrombin III regulates complement activity in vitro.

J M Weiler1, R J Linhardt

  • 1Iowa City VA Medical Center, IA.

Journal of Immunology (Baltimore, Md. : 1950)
|June 1, 1991
PubMed
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Antithrombin III, a protein, regulates the complement system (C) pathways. When combined with heparin, its ability to inhibit C pathways is enhanced, suggesting a key role in immune regulation.

Area of Science:

  • Immunology
  • Biochemistry

Background:

  • Heparin inhibits coagulation via antithrombin III.
  • Heparin alone can regulate the complement system (C).

Purpose of the Study:

  • To investigate antithrombin III's role in regulating classical and alternative C pathways.
  • To determine the synergistic effects of antithrombin III and heparin on C regulation.

Main Methods:

  • Dose-response experiments using purified human antithrombin III and heparin.
  • Assessing inhibition of classical and alternative pathway convertases.
  • Evaluating effects on terminal lysis and specific C pathway intermediates.

Main Results:

  • Antithrombin III alone inhibited classical (60%) and alternative (42%) C pathway convertase generation.

Related Experiment Videos

  • Antithrombin III and heparin demonstrated dose-dependent synergistic inhibition of C pathways.
  • Antithrombin III inhibited factor B and potentially factor D activity.

    • Conclusions:

    • Antithrombin III effectively regulates the complement system.
    • The combination of antithrombin III and heparin significantly enhances C pathway inhibition.
    • Antithrombin III may be crucial for in vivo complement regulation, especially with heparin.