Related Experiment Video
Updated: Jun 14, 2026
Photodynamic Therapy with Blended Conducting Polymer/Fullerene Nanoparticle Photosensitizers
Published on: October 28, 2015
Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes
S V Prylutska1, I I Grynyuk, K O Palyvoda
1Taras Shevchenko National University, Kyiv, Ukraine.
Aim:
To estimate the viability of normal and transformed T-lymphocytes after UV/Vis irradiation in the presence of pristine fullerenes C(60).
Methods:
Thymocytes were isolated from Wistar rats' thymus. Murine leukemia L1210 and human lymphoma Jurkat cells were used in this study. Mercury-vapor lamp was used for fullerenes C(60) photoexcitation. Cytotoxicity was etermined by MTT assay. Changes in cell morphology were monitored by phase-contrast light microscopy.
Results:
fullerenes C(60) exhibit cytotoxic effect against transformed T-lymphocytes when combined with UV/Vis irradiation (320-600 nm). Photoinduced effect was enhanced with the increasing of irradiation time period and C(60) concentration, cell death was registered after 24 hours incubation. Fullerenes C(60) photocytotoxicity against normal T-lymphocytes (thymocytes) was not observed.
Conclusion:
The present study suggests that pristine fullerenes C(60) have the potential to be an effective photosensitizer and exhibit cytotoxic effect on transformed T-cells in vitro .
