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Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha
O A Kovaleva1, N O Bezdenezhnykh, T T Glazko
1R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kiev 03022, Ukraine.
Aim:
To estimate the effect of long-term IFN treatment of human non-small-cell lung cancer cell line A-549 on their karyotype characteristics and on the clonal structure of cell population.
Methods:
Cytogenetic research was performed by standard methods using routine and differential staining. Cytogenetic characteristics were estimated per 1000 cells (ppm, (per thousand)).
Results:
Cytogenetic analysis of IFN-modified A-549 human lung cancer cells had demonstrated far-going changes in their population structure. It was shown that long term cultivation with IFN altered the chromosome modal class of A-549 cells, induced the domination of hromosomes with certain molecular markers: the number of metaphases with der (6) t (6; 1) chromosomal rearrangement increased significantly (from 6% to 80%, p
Conclusion:
Long-term IFN effect results in alterations of cytogenetic properties of A-549 human lung cancer cells.