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Related Experiment Video

Updated: Jun 14, 2026

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
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3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells

Published on: March 28, 2025

Engineering a novel three-dimensional contractile myocardial patch with cell sheets and decellularised matrix.

Hiroki Hata1, Antonia Bär, Suzanne Dorfman

  • 1Leibniz Research Laboratories for Biotechnology and Artificial Organs, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover, Germany. Hata.Hiroki@mh-hannover.de

European Journal of Cardio-Thoracic Surgery : Official Journal of the European Association for Cardio-Thoracic Surgery
|March 26, 2010
PubMed
Summary

This study developed thicker, contracting myocardial patches for cardiac repair by layering cardiomyocyte sheets onto seeded small intestinal submucosa. The resulting constructs demonstrated synchronized contractions in a defined direction, advancing tissue engineering for cardiac grafts.

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Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing

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Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Tissue Engineering

Background:

  • Generating functional myocardial patches for cardiac repair faces challenges in maintaining contraction in thicker constructs.
  • Neonatal rat cardiomyocytes (CMs) seeded on decellularised porcine small intestinal submucosa (SIS) have previously shown directional contraction.

Purpose of the Study:

  • To create a thicker, functional myocardial patch with sustained contraction.
  • To investigate the combination of CM cell sheets with CM-seeded SIS for enhanced cardiac tissue constructs.

Main Methods:

  • Decellularised porcine SIS was seeded with neonatal rat CMs.
  • CM sheets were prepared and layered (one or three sheets) onto the seeded SIS.
  • Constructs were cultured for 10 days, and contraction and histology were analyzed.

Main Results:

  • Stacked CM sheets and seeded SIS constructs exhibited spontaneous and synchronous contractions.
  • A high percentage of constructs showed defined contraction directionality, parallel to the longitudinal axis (70-83%).
  • The thickness of the constructs increased to approximately 800 μm with three layered sheets.

Conclusions:

  • Combining layered CM sheets with CM-seeded SIS successfully generated a 3D myocardial patch with directional contraction.
  • This approach represents a significant step towards developing vascularized, contractile myocardial grafts for cardiac regeneration.