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Laboratory host systems for extraneous agent testing in avian live virus vaccines: problems encountered

Hans-C Philipp1, Ines Kolla

  • 1Lohmann Tierzucht GmbH, Veterinary Laboratory, Abschnede 64, 27472 Cuxhaven, Germany. philipp@ltz.de

Biologicals : Journal of the International Association of Biological Standardization
|March 26, 2010
PubMed

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Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...