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Updated: Jun 14, 2026

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
Published on: June 25, 2017
[High glucose up-regulates GRK2 gene expression in H9C2 cardiomyoblasts in vitro]
Hong-mei Chen1, Qiu-xiong Lin, Hong-hong Tan
1Department of Endocrinology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China. ch_may@163.com
Objective:
To study the effect of high glucose on GRK2 gene expression in H9C2 cardiomyoblasts in vitro.
Methods:
H9C2 cardiomyoblasts were cultured for 72 h in the presence of 0, 5.5, 12.5, 25 or 33 mmol/L glucose (with the osmotic pressure adjusted with monnitol). Semi-quantitative detection of GRK2 gene expression in H9C2 cardiomyoblasts was carried out using RT-PCR and phosph-Akt (Ser473) protein level was measured by Western blotting.
Results:
Glucose in the culture medium (5.5 to 33 mmol/L) concentration-dependently increased the mRNA expression of GRK2 concentration and decreased phosphorylation Akt (ser473) level in in H9C2 cardiomyoblasts.
Conclusion:
Increased GRK2 gene expression may play an important role in cardiac dysfunction in diabetes.
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