Related Experiment Video
Updated: Jun 14, 2026
Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
Published on: March 18, 2019
[Neuronal differentiation of PC12 cells induced by human periodontal ligament fibroblast-conditioned medium]
Juan Xu1, Hong-chen Liu, Lu-lu Xu
1Department of Stomatology, General Hospital of PLA, Beijing, China. newxj@hotmail.com
Objective:
To examine whether human periodontal ligament fibroblasts (hPDLF) express neural growth factor (NGF).
Methods:
PC12 cells were cultured in hPDLF-conditioned medium to evaluate the secretion of NGF by hPDLF.
Results:
hPDLF-conditioned medium resulted in neuronal differentiation and neurite outgrowth from PC12 cells. Only a small percentage of the cells grown in anti-NGF antiserum and DMEM expressed neuritis.
Conclusion:
hPDLF are capable of synthesizing NGF and secreting it into the medium as the signaling molecules for innervation during the healing of the periodontal tissue.
