Cytotoxic effects of hydrogen sulfide on pulmonary alveolar macrophages in rats

Respiratory rates (basal and zymosan-stimulated) and cell viability were monitored in pulmonary alveolar macrophages (PAM) from rats exposed to 0, 70, 280, and 560 mg/m3 (0, 50, 200, and 400 ppm) hydrogen sulfide (H2S) gas for 4 h. Zymosan-stimulated respiratory rates were markedly reduced in PAM collected from rats exposed to 280 and 560 mg/m3 H2S; however, their basal respiratory rates were not affected. Significant decrease in cell viability was also observed in samples from 560 mg/m3 H2S-treated rats, but it remained high and unchanged in other treatments. In vitro incubation of PAM from control rats with sulfide (a precursor of H2S) and its two oxidation products, sulfite and sulfate, showed that sulfide was markedly more inhibitory to both respiratory rates than sulfite or sulfate. These treatments did not affect cell viability.