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Related Concept Videos

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...

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Related Experiment Video

Updated: Jun 14, 2026

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
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Published on: March 7, 2018

Measuring membrane protein interactions using optical biosensors.

Joseph Rucker1, Candice Davidoff, Benjamin J Doranz

  • 1Integral Molecular, Inc, Philadelphia, PA, USA. jrucker@integralmolecular.com

Methods in Molecular Biology (Clifton, N.J.)
|March 26, 2010
PubMed
Summary

Virus-like particles called Lipoparticles present membrane proteins for study. This method facilitates characterization of antibody interactions with these challenging targets using biosensors.

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Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
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Published on: August 6, 2018

Related Experiment Videos

Last Updated: Jun 14, 2026

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
10:41

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay

Published on: March 7, 2018

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

Area of Science:

  • Biochemistry
  • Structural Biology
  • Biophysics

Background:

  • Membrane proteins like GPCRs and ion channels are crucial drug targets but difficult to study due to their lipid bilayer requirement.
  • Existing methods for biophysical manipulation of membrane proteins are often limited.
  • Novel approaches are needed to overcome these challenges in drug discovery and research.

Purpose of the Study:

  • To introduce and validate the use of virus-like particles (Lipoparticles) for presenting membrane proteins.
  • To demonstrate a method for characterizing membrane protein interactions using Lipoparticles on optical biosensors.
  • To enable detailed kinetic, affinity, and specificity analysis of antibody-membrane protein interactions.

Main Methods:

  • Development and application of Lipoparticles for presenting diverse membrane proteins.
  • Utilizing optical biosensors, specifically the BioRad ProteOn XPR36 system.
  • Employing surface plasmon resonance (SPR) detection for interaction analysis.

Main Results:

  • Lipoparticles successfully present functional membrane proteins in a format amenable to biosensor analysis.
  • Characterization of antibody binding kinetics, affinity, and specificity was achieved using SPR.
  • The Lipoparticle approach provides a robust platform for studying membrane protein interactions.

Conclusions:

  • Lipoparticles offer a versatile and effective strategy for overcoming the challenges of membrane protein biophysics.
  • This method significantly advances the study of membrane protein interactions, crucial for therapeutic development.
  • The described biosensor-based approach using Lipoparticles enhances the characterization of antibody-target engagement.