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Updated: Jun 14, 2026
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
Published on: July 29, 2018
Isolation procedure and characterization of multipotent adult progenitor cells from rat bone marrow
Kartik Subramanian1, Martine Geraerts, Karen A Pauwelyn
1Department of Chemical Engineering and Materials Science, Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA.
Abstract:
Multipotent adult progenitor cells (MAPCs) are adult stem cells derived from the bone marrow of mouse and rat and were described for the first time in 2002 (Jiang et al., Nature 418:41-49, 2002), and subsequently (Breyer et al., Exp Hematol 34:1596-1601, 2006; Jiang et al., Exp Hematol 30:896-904, 2002; Ulloa-Montoya et al., Genome Biol 8:R163, 2007). The capacity of rodent MAPC to differentiate at the single-cell level into some of the cell types of endoderm, mesoderm, and neuroectoderm germ layer lineages makes them promising candidates for the study of developmental processes. MAPC are isolated using adherent cell cultures and are selected based on morphology after a period of about 8-18 weeks. Here, we describe a step-by-step reproducible method to isolate rat MAPC from fetal and adult bone marrow. We elaborate on several aspects of the isolation protocol including, cell density and medium components, and methods for selecting and obtaining potential MAPC clones and their characterization.
Insights
This study details a reproducible method for isolating multipotent adult progenitor cells (MAPCs) from rat bone marrow. These cells hold promise for developmental biology research due to their differentiation potential.
Area of Science:
- Stem Cell Biology
- Developmental Biology
- Regenerative Medicine
Background:
- Multipotent adult progenitor cells (MAPCs) are adult stem cells with differentiation potential into multiple germ layers.
- MAPCs were first identified in rodents in 2002 and have since been a focus of research.
- Their ability to differentiate makes them valuable for studying developmental processes.
Purpose of the Study:
- To describe a reproducible, step-by-step method for isolating rat MAPCs.
- To optimize the isolation protocol for MAPCs from both fetal and adult rat bone marrow.
- To detail the selection and characterization of MAPC clones.
Main Methods:
- Isolation of MAPCs from rat bone marrow using adherent cell cultures.
- Optimization of cell density and culture medium components.
- Selection and characterization of potential MAPC clones based on morphology and differentiation capacity.
Main Results:
- A detailed and reproducible protocol for rat MAPC isolation is presented.
- Key aspects of the isolation, including cell density and medium, are elaborated.
- Methods for selecting and characterizing MAPC clones are described.
Conclusions:
- The described method provides a reliable way to obtain rat MAPCs.
- This protocol facilitates further research into MAPC biology and potential therapeutic applications.
- Rat MAPCs are valuable tools for investigating developmental processes and stem cell differentiation.
