Related Experiment Video

Updated: Jun 14, 2026

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
07:20

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Published on: June 14, 2018

Altering the specificity of NK:target cell interactions by genetic manipulation of NK receptor expression on primary

Jimmy Tran1, Sajid Mahmood, James R Carlyle

  • 1Room 417 Apotex Centre, Department of Immunology, University of Manitoba, 750 McDermont Avenue, Winnipeg, MB, Canada R3E 0T5.

Vaccine
|March 27, 2010
PubMed

A balance of signals generated via stimulatory and inhibitory NK receptors determines both target cell specificity and the outcome of NK-target cell interactions. The feasibility of introducing naturally occurring or genetically engineered chimeric NK receptors at the effector cell level may prove useful in NK cell-based immunotherapies. Here, we utilized a previously established lentiviral transduction system to over-express a model NKR-P1B inhibitory receptor on primary mouse NK cells. These genetically engineered NK cells became more sensitive to inhibitory signals delivered by target cells expressing the cognate NKR-P1B ligand, Ocil/Clr-b. This study demonstrated the utility of lentiviral vectors as a means to stably manipulate the target cell specificity of primary NK cells.

More Related Videos

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells08:42

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

Published on: January 15, 2018

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons09:40

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

Published on: June 30, 2022

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...