Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 14, 2026

Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture
10:55

Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture

Published on: January 11, 2016

Modularly assembled porous cell-laden hydrogels.

Bo Liu1, Yang Liu, Andrew K Lewis

  • 1Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.

Biomaterials
|March 27, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineered protein-small molecule conjugates empower selective enzyme inhibition.

Cell chemical biology·2021
Same author

Death Receptor 5 Activation Is Energetically Coupled to Opening of the Transmembrane Domain Dimer.

Biophysical journal·2017
Same author

Minimal Nucleation State of α-Synuclein Is Stabilized by Dynamic Threonine-Water Networks.

ACS chemical neuroscience·2017
Same author

An Innovative High-Throughput Screening Approach for Discovery of Small Molecules That Inhibit TNF Receptors.

SLAS discovery : advancing life sciences R & D·2017
Same author

Piecing it together: Unraveling the elusive structure-function relationship in single-pass membrane receptors.

Biochimica et biophysica acta. Biomembranes·2017
Same author

Death Receptor 5 Networks Require Membrane Cholesterol for Proper Structure and Function.

Journal of molecular biology·2016

Researchers assembled porous, cell-laden hydrogels for tissue engineering. Star-shaped microgels create highly porous, permeable constructs suitable for centimeter-sized tissue products with enhanced cell viability.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Hydrogel Engineering

Background:

  • 3D tissue engineering faces challenges with current scaffold limitations.
  • Developing porous, cell-laden hydrogels is crucial for effective tissue regeneration.
  • Modular assembly offers a promising approach to overcome these limitations.

Purpose of the Study:

  • To develop a method for modularly assembling porous, cell-laden hydrogels for 3D tissue engineering.
  • To investigate the influence of microgel morphology on the properties of the assembled constructs.
  • To assess the suitability of these constructs for large-scale tissue engineering applications.

Main Methods:

  • Photolithographic fabrication of poly(ethylene glycol) diacrylate microgels.

More Related Videos

Interlinked Macroporous 3D Scaffolds from Microgel Rods
07:32

Interlinked Macroporous 3D Scaffolds from Microgel Rods

Published on: June 16, 2022

Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology
05:21

Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology

Published on: May 16, 2022

Related Experiment Videos

Last Updated: Jun 14, 2026

Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture
10:55

Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture

Published on: January 11, 2016

Interlinked Macroporous 3D Scaffolds from Microgel Rods
07:32

Interlinked Macroporous 3D Scaffolds from Microgel Rods

Published on: June 16, 2022

Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology
05:21

Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology

Published on: May 16, 2022

  • Modular assembly of microgels using a polypeptide-based cross-linker via Michael-type addition.
  • Characterization of construct porosity, permeability, and pore interconnectivity based on microgel shape (star, circle, square).
  • In vitro culture of cells within centimeter-sized constructs under perfusion.

    • Main Results:

    • Star-shaped microgels yielded constructs with higher porosity, permeability, and pore interconnectivity compared to circle- and square-shaped microgels.
    • A correlation was established between microgel morphology and the macroscopic properties of the assembled constructs.
    • Centimeter-sized, cell-laden constructs maintained cell viability under perfusion culture.
    • The assembled constructs demonstrated advantages of both preformed scaffolds and in situ forming hydrogels.

    Conclusions:

    • Modular assembly of microgels provides a bottom-up strategy to control the properties of porous hydrogel constructs.
    • The developed method enables the creation of large, centimeter-sized, cell-laden constructs suitable for tissue engineering.
    • This approach offers a versatile platform for developing 3D tissue engineered products with enhanced mass transfer and cell protection.