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Updated: Jun 14, 2026
Culture of myeloid dendritic cells from bone marrow precursors
Published on: July 25, 2008
Transformation of dendritic cells from plasmacytoid to myeloid in a leukemic plasmacytoid dendritic cell line
Norihiro Watanabe1, Miwako Narita, Akie Yamahira
1Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University, Niigata, Japan.
Abstract:
We investigated the transformation from pDCs to mDCs in a pDC line (PMDC05) which was established from a patient with pDC leukemia in our laboratory. PMDC05 cells were separated into two fractions according to the expression of BDCA1 and CD123. BDCA1(-)CD123(+) cells were found to be pDC-like cells by their morphology, surface phenotypes, mRNA expression and the function. In addition, BDCA1(-)CD123(+) cells were demonstrated to have a proliferating capacity and revealed the ability to transform to BDCA1(+)CD123(-) cells which showed mDC-like properties. Our data demonstrated the possibility of transformation from pDCs to mDCs in human DC lineage.
Insights
This study shows that plasmacytoid dendritic cells (pDCs) can transform into myeloid dendritic cells (mDCs). This discovery offers new insights into human dendritic cell lineage and potential therapeutic targets.
Area of Science:
- Immunology
- Cell Biology
- Hematology
Background:
- Plasmacytoid dendritic cells (pDCs) and myeloid dendritic cells (mDCs) are crucial immune cells.
- Understanding the plasticity and differentiation pathways of dendritic cells is vital for immunology.
Purpose of the Study:
- To investigate the potential transformation of pDCs into mDCs.
- To characterize the properties of cells involved in this potential DC lineage transformation.
Main Methods:
- Establishment and characterization of a pDC leukemia cell line (PMDC05).
- Separation of PMDC05 cells based on BDCA1 and CD123 expression.
- Analysis of cell morphology, surface phenotypes, mRNA expression, and function.
Main Results:
- BDCA1(-)CD123(+) cells exhibited pDC-like characteristics.
- These pDC-like cells demonstrated proliferative capacity.
- BDCA1(-)CD123(+) cells transformed into BDCA1(+)CD123(-) cells with mDC-like properties.
Conclusions:
- The study provides evidence for the possibility of pDC to mDC transformation in humans.
- This finding suggests significant plasticity within the human dendritic cell lineage.
- The PMDC05 cell line serves as a valuable model for studying DC differentiation.
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