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Updated: Jun 14, 2026

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays
Published on: February 4, 2021
Common structural motifs for the regulation of divergent class II myosins
Susan Lowey1, Kathleen M Trybus
1Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, Vermont 05405, USA. susan.lowey@uvm.edu
Abstract:
This minireview focuses on structural studies that have provided insights into our current understanding of thick filament regulation in muscle. We describe how different domains in the myosin molecule interact to produce an inactive "off" state; included are head-head and head-rod interactions, the role of the regulatory light chain, and the significance of the alpha-helical coiled-coil rod in regulation. Several of these interactions have now been visualized in a wide variety of native myosin filaments, testifying to the generality of these structural motifs across the phylogenetic tree.
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