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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
Expression of CD44v6 as matrix-associated ectodomain in the bone development
Kosei Nakajima1, Kazumi Taniguchi, Ken-ichiro Mutoh
1Laboratory of Veterinary Anatomy, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
The Journal of Veterinary Medical Science
|March 27, 2010
Summary
CD44v6 is expressed during embryonic bone development, potentially aiding matrix formation. However, CD44v6 is not detected in adult bone fracture healing, suggesting a distinct role in development versus repair.
Area of Science:
- Biochemistry
- Developmental Biology
- Cell Biology
Background:
- The CD44 family comprises transmembrane glycoproteins involved in cell adhesion.
- CD44v6, a CD44 variant, is implicated in osteosarcoma metastasis.
- Cancer metastasis often involves reversion to immature cellular forms.
Purpose of the Study:
- To investigate the expression of CD44v6 and CD44 standard (CD44st) during bone development.
- To examine CD44v6 and CD44st expression in experimentally induced fracture models.
Main Methods:
- Immunohistochemical analysis of CD44v6 and CD44st expression.
- Study of fetal, young adult, and fracture-healing rat bone tissues.
Main Results:
- CD44st expression was observed in osteoblasts and osteocytes from fetal to young ages.
- CD44v6 showed intense bone matrix expression in fetal stages, exceeding CD44st.
- CD44v6 was not detected in osteoblasts or bone matrix during fracture healing, while CD44st was present.
Conclusions:
- CD44v6 expression during embryonic development suggests a role in bone matrix formation.
- CD44v6 does not appear to be involved in the process of fracture healing.
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