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Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
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Enhanced MALDI-TOF MS analysis of phosphopeptides using an optimized DHAP/DAHC matrix

Junjie Hou1, Zhensheng Xie, Peng Xue

  • 1Proteomic Platform and National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Journal of Biomedicine & Biotechnology
|March 27, 2010
PubMed
Summary

A new matrix combination of 2,6-dihydroxyacetophenone (DHAP) and diammonium hydrogen citrate (DAHC) significantly enhances phosphopeptide detection in MALDI-TOF-MS. This optimized matrix improves sensitivity and sample homogeneity for identifying phosphorylation sites.

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