Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Spatial analysis of sheep fascioliasis in rural communities of the Southern highlands of Peru.

BMC veterinary research·2026

Same author

Efficacy of two triclabendazole regimens for treating human chronic fasciolosis and a controlled efficacy trial in sheep using human-isolate metacercariae in Cajamarca, Peru.

Parasitology international·2026

Same author

Mitochondrial genome analysis supports zoonotic transmission of triclabendazole-resistant human fascioliasis in Peru.

One health (Amsterdam, Netherlands)·2025

Same author

Dichroism of coupled multipolar plasmonic modes in twisted triskelion stacks.

Nanophotonics (Berlin, Germany)·2025

Same author

Helminth/Protozoan Coinfections in Chronic Fascioliasis Cases in Human Hyperendemic Areas: High Risk of Multiparasitism Linked to Transmission Aspects and Immunological, Environmental and Social Factors.

Tropical medicine and infectious disease·2025

Same author

National Seroprevalence and Risk Factors of Bluetongue Virus in Domestic Ruminants of Peru.

Transboundary and emerging diseases·2025

Search research articles

Related Experiment Video

Updated: Jun 14, 2026

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates

Cell Patterning on Photolithographically Defined Parylene-C: SiO₂ Substrates

Published on: March 7, 2014

Biomolecule patterning on analytical devices: a microfabrication-compatible approach.

Guillaume Suárez¹, Neil Keegan, Julia A Spoors

¹Diagnostic and Therapeutic Technologies, The Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, UK. guillaume.suarez@epfl.ch

Langmuir : the ACS Journal of Surfaces and Colloids

|March 30, 2010

Summary

This study introduces a novel method for 3-D biomolecule patterning on silicon devices, combining microfabrication with simple biomolecule loading. This approach overcomes compatibility issues, enabling advanced biological assays.

More Related Videos

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

Bridging the Bio-Electronic Interface with Biofabrication

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

Related Experiment Videos

Last Updated: Jun 14, 2026

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates

Cell Patterning on Photolithographically Defined Parylene-C: SiO₂ Substrates

Published on: March 7, 2014

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

Bridging the Bio-Electronic Interface with Biofabrication

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

Area of Science:

Biomaterials Science
Microfluidics
Analytical Chemistry

Background:

Standard methods for biomolecule patterning on silicon devices face challenges integrating biological components with microfabrication processes.
Existing sol-gel techniques often involve introducing biomolecules during material synthesis, leading to compatibility issues and reduced biological activity.

Purpose of the Study:

To develop a novel methodology for 3-D biomolecule patterning on silicon-based analytical devices.
To reconcile 3-D biological functionalization with standard microfabrication resist lift-off techniques.
To circumvent compatibility issues between biological materials and microfabrication processes.

Main Methods:

A two-stage approach was developed: first, patterning micrometer/submicrometer polycondensate scaffolds using microfabrication tools.
Second, loading native biomolecules into the pre-formed scaffolds via incubation under biologically compatible conditions.
Scaffolds were generated on silicon substrates using the polycondensation of aminopropyltriethoxy silane (APTES), compatible with resist mask lithography.

Main Results:

The developed method successfully created 3-D scaffold structures with high porosity and primary amine content.
These scaffolds effectively loaded proteins and nucleic acid sequences, maintaining high biological activity.
The biopatterning approach was integrated into silicon analytical device fabrication, demonstrated by successful immunoassays and nucleic acid assays.

Conclusions:

The novel two-stage patterning methodology enables 3-D biomolecule functionalization on silicon devices, overcoming traditional compatibility challenges.
This approach allows for the introduction of native biomolecules downstream of microfabrication, preserving their activity.
The technique is suitable for fabricating advanced silicon analytical devices for various biological assays.