Related Experiment Video
Updated: Jun 14, 2026
High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
Published on: June 11, 2013
Implementation and validation of the Teleshake unit for DNA IQ robotic extraction and development of a large volume
Jennifer C Grubb1, Katie M Horsman-Hall, Karen L V Sykes
1Virginia Department of Forensic Science, Forensic Biology, 700 North 5th Street, Richmond, VA 23219, USA.
Abstract:
Automated platforms used for forensic casework sample DNA extraction need to be versatile to accommodate a wide variety of sample types, thus protocols frequently need modification. In this study, DNA IQ methods previously developed for the Biomek 2000 Automation Workstation were adapted for the Teleshake Unit using normal volumes and all deepwell extraction, and a large volume DNA IQ method developed. DNA purification without detectable contamination of adjacent reagent blanks is reported in the extraction of tissue samples containing several micrograms of DNA. Sensitivity and contamination studies demonstrated similar performance with the manual organic extraction method for bloodstain dilution samples. Mock casework samples demonstrated the effectiveness of the Teleshake and Teleshake large volume methods. Because of the performance and increased versatility of the DNA IQ extraction with these modifications, the Teleshake Unit has been implemented in both normal and large volume automated DNA extractions at the Virginia Department of Forensic Science.
