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Selenium-enriched milk proteins and selenium yeast affect selenoprotein activity and expression differently in mouse
Ying Hu1, Graeme H McIntosh, Richard K Le Leu
1Flinders Centre for Cancer Prevention and Control, Flinders University of South Australia, Bedford Park 5042, Australia. ying.hu@flinders.edu.au
The British Journal of Nutrition
|March 30, 2010
Summary
Dietary selenium (Se) affects selenoprotein levels differently. Dairy-Se increased selenoprotein P and gastrointestinal glutathione peroxidase-2 mRNA in mouse colons, while Se yeast boosted glutathione peroxidase-1. These findings suggest distinct mechanisms for Se
Area of Science:
- Nutritional Biochemistry
- Molecular Biology
- Cancer Prevention
Background:
- Dietary selenium (Se) plays a crucial role in human health, influencing selenoprotein activity and potentially cancer risk.
- Different forms of dietary selenium, such as Se-enriched milk protein (dairy-Se) and Se yeast (yeast-Se), may have varying effects on Se status and chemoprevention.
- Understanding the differential impact of various Se supplements on selenoprotein expression in the colon is essential for optimizing dietary strategies.
Purpose of the Study:
- To compare the effects of dairy-Se and yeast-Se on selenoprotein activity and expression in the mouse colon.
- To investigate the dose-dependent effects of dairy-Se on specific selenoproteins.
- To elucidate the molecular mechanisms underlying the chemopreventive potential of different selenium forms.
Main Methods:
- Mice were fed control, dairy-Se (0.5 and 1 ppm), or yeast-Se (1 ppm) diets for 4 weeks.
- Analysis of cytosolic glutathione peroxidase-1 (GPx-1) activity and mRNA levels of selenoprotein P (SeP), GPx-1, gastrointestinal glutathione peroxidase-2 (GPx-2), and thioredoxin reductase-1 (TrxR-1) in mouse colons.
- Statistical analysis to determine significant differences in selenoprotein expression and activity between dietary groups.
Main Results:
- Dairy-Se supplementation (1 ppm) significantly increased SeP and GPx-2 mRNA in a dose-dependent manner, without affecting GPx-1.
- Yeast-Se (1 ppm) significantly increased GPx-1 mRNA and activity, but did not impact GPx-2 mRNA.
- Neither dairy-Se nor yeast-Se affected TrxR-1 mRNA levels, indicating differential regulation of selenoproteins by various Se forms.
Conclusions:
- Selenoprotein expression in the mouse colon is differentially regulated by the form of selenium supplementation.
- The observed up-regulation of colonic GPx-2 and SeP by dairy-Se may contribute to its previously demonstrated chemopreventive effects against colorectal cancer.
- These findings highlight the importance of considering the specific Se source when evaluating its health benefits and chemopreventive potential.
