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A simple method for the simultaneous decoloration and deproteinization of crude levan extract from Paenibacillus
Jun Liu1, Jianguang Luo, Yi Sun
1College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
Abstract:
A simple method for the simultaneous decoloration and deproteinization of crude levan extract from the endophytic bacterium Paenibacillus polymyxa EJS-3 was developed through static and dynamic adsorption tests of macroporous resins. S-8 resin demonstrated the highest decoloration and deproteinization ratios among various resins tested. Under optimized static adsorption conditions (pH 6.0, 35 degrees C and adsorption time of 70 min), the ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin were 76.8%, 78.9% and 69.0%, respectively. Under optimized dynamic adsorption condition (flow rate of 2 BV/h, 160 ml of 2.5mg/ml crude levan extract), higher ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin (84.6%, 91.7% and 81.3%, respectively) were observed. The method developed will provide a potential approach for large-scale production of levan from P.polymyxa EJS-3.
