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Syngas fermentation in a 100-L pilot scale fermentor: design and process considerations
Dimple K Kundiyana1, Raymond L Huhnke, Mark R Wilkins
1214c Ag Hall, Oklahoma State University, Stillwater, OK 74078-6021, USA.
Journal of Bioscience and Bioengineering
|March 30, 2010
Summary
Scaling up syngas fermentation using Clostridium strain P11 in a pilot-scale fermentor significantly improved ethanol production. This research demonstrates enhanced efficiency and the formation of valuable compounds through optimized anaerobic fermentation.
Area of Science:
- Biotechnology
- Industrial Microbiology
- Chemical Engineering
Background:
- Syngas fermentation offers advantages over chemical catalysis, including biocatalyst specificity, reduced energy costs, and improved carbon efficiency.
- Scaling up fermentation processes from laboratory to pilot scale is essential for commercialization.
Purpose of the Study:
- To install and commission a pilot-scale fermentor.
- To scale up Clostridium strain P11 fermentation from a 7.5-L to a 100-L fermentor.
- To evaluate the impact of anaerobic conditions and syngas mass transfer on fermentation efficiency and product formation.
Main Methods:
- Installation and commissioning of a 100-L pilot-scale fermentor.
- Conducting anaerobic batch fermentations with continuous syngas supply.
- Optimizing syngas mass transfer coefficient to enhance substrate utilization and product yield.
Main Results:
- Achieved a six-fold increase in ethanol concentration compared to serum bottle fermentation.
- Successfully produced commercially relevant compounds including isopropyl alcohol, acetic acid, and butanol.
- Demonstrated the feasibility of pilot-scale syngas fermentation under optimized anaerobic conditions.
Conclusions:
- Pilot-scale syngas fermentation is a viable strategy for enhanced biofuel and chemical production.
- Optimizing mass transfer and maintaining strict anaerobic conditions are critical for maximizing yield and product diversity.
- The scaled-up process shows significant potential for the commercial production of valuable chemicals from syngas.
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