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Related Experiment Video

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

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Published on: October 18, 2022

Complexity of expressed CHIR genes.

Birgit C Viertlboeck¹, Claudia M Gick, Ramona Schmitt

¹Institute for Animal Physiology, University of Munich, Veterinaerstr. 13, 80539 Munich, Germany. Birgit.Viertlboeck@lmu.de

Developmental and Comparative Immunology

|March 30, 2010

Summary

Chicken leukocyte receptor complex (LRC) research reveals high variability in expressed chicken Ig-like receptors (CHIR) with two Ig domains. Few CHIR sequences are conserved across different chicken strains, indicating significant genetic polymorphism.

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Area of Science:

Immunology
Genomics
Comparative Biology

Background:

The chicken leukocyte receptor complex (LRC) has a significantly higher number of chicken Ig-like receptor (CHIR) genes than mammalian LRC.
Limited information exists on the diversity and expression patterns of CHIR genes, particularly those with two Ig domains.

Purpose of the Study:

To investigate the number and variability of expressed CHIR genes with two Ig domains (CHIR2D) in different chicken strains.
To compare CHIR2D sequences across chickens with distinct MHC haplotypes.

Main Methods:

Used oligonucleotides targeting conserved regions of Ig1 and Ig2 domains.
Analyzed PBMC mRNA from two chickens (M11, R11) with different MHC haplotypes.
Sequenced and compared CHIR2D amino acid sequences from M11, R11, and a genomic dataset from LSL chickens.

Main Results:

Identified 98 unique CHIR2D amino acid sequences in M11 and 70 in R11.
Found only one identical amino acid sequence shared across all three chicken strains.
Detected high variability at three amino acid positions and evidence of positive selection.

Conclusions:

Expressed CHIR2D genes exhibit unusual polymorphism in chickens.
There is limited sequence conservation of expressed CHIR2D among different chicken strains.
The findings highlight significant genetic diversity within the chicken immune system's receptor repertoire.