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Updated: Jun 14, 2026

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening
Published on: November 20, 2018
In vitro methods to assess drug precipitation
1Johnson & Johnson Pharmaceutical Research & Development, LLC., 145 King of Prussia Road, Radnor, PA 19087, USA. wdai3@its.jnj.com
Drug precipitation in vivo can reduce medication effectiveness. This review covers new in vitro assays that efficiently screen drug precipitation potential for intravenous and oral formulations, aiding drug development.
Area of Science:
- Pharmaceutical Sciences
- Drug Delivery
- Biopharmaceutics
Background:
- In vivo drug precipitation after administration can decrease therapeutic efficacy.
- Current in vivo assays for drug precipitation are limited in practicality.
- Efficient in vitro methods are needed for early-stage drug precipitation assessment.
Purpose of the Study:
- To review available in vitro precipitation screening assays.
- To cover assays for both intravenous and oral drug formulations.
- To provide a guide for assessing drug precipitation potential.
Main Methods:
- Literature review of in vitro precipitation screening assays.
- Analysis of assay applicability for intravenous formulations.
- Analysis of assay applicability for oral formulations.
Main Results:
- Several in vitro assays for drug precipitation have been developed.
- These assays offer efficient screening of precipitation potential.
- The review categorizes assays based on formulation type (IV, oral).
Conclusions:
- In vitro precipitation assays are valuable tools for pharmaceutical development.
- These assays help predict and mitigate in vivo precipitation issues.
- Further development and validation of these assays are ongoing.
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