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Efficient electrotransformation of Bacteroides fragilis

Minoru Ichimura1, Haruyuki Nakayama-Imaohji, Shin Wakimoto

  • 1Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.

Applied and Environmental Microbiology
|March 30, 2010
PubMed
Summary

Optimized electroporation methods enhance Bacteroides fragilis transformation using specific plasmids and growth conditions. This facilitates efficient gene disruption for genetic manipulation of Bacteroides species.

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