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Expression in Escherichia coli of the native cyt1Aa from Bacillus thuringiensis subsp. israelensis
Vladislav Sazhenskiy1, Arieh Zaritsky, Mark Itsko
1Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel.
Applied and Environmental Microbiology
|March 30, 2010
Abstract:
The gene cyt1Aa is one of the genes in the complex determining the mosquito larvicidity of Bacillus thuringiensis subsp. israelensis. Previous cloning in Escherichia coli resulted in a 48-bp addition upstream, encoding a chimera. Here, cyt1Aa was recloned without the artifact, and its toxicity against Aedes aegypti larvae and host E. coli cells was retested.
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