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Related Experiment Video

Updated: Jun 14, 2026

Primary Human Bronchial Epithelial Cells Grown from Explants
14:32

Primary Human Bronchial Epithelial Cells Grown from Explants

Published on: March 26, 2010

Primary human bronchial epithelial cells grown from explants.

Asma Yaghi1, Aisha Zaman, Myrna Dolovich

  • 1Medicine, Faculty of Health Sciences, McMaster University. ayaghi@stjosham.on.ca

Journal of Visualized Experiments : Jove
|March 30, 2010
PubMed
Summary

This study details a method for culturing primary human bronchial epithelial cells from lung tissue. These cells are vital for disease modeling and drug testing, providing an abundant source for research.

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Area of Science:

  • Cell Biology
  • Respiratory Medicine
  • Tissue Engineering

Background:

  • Primary human bronchial epithelial cells are crucial for disease modeling and pharmacological studies.
  • Existing methods for obtaining these cells can be limiting for research needs.

Purpose of the Study:

  • To describe a detailed, reproducible method for isolating and culturing primary human bronchial epithelial cells from surgical lung tissue.
  • To establish an abundant and consistent source of these cells for research applications.

Main Methods:

  • Bronchial tissue segments are obtained from lung resections with ethical approval.
  • Tissue explants are cultured on collagen, fibronectin, and BSA-coated plates in specialized medium.
  • Cells are expanded in flasks and can be differentiated at the air-liquid interface.

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Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells
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Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

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Last Updated: Jun 14, 2026

Primary Human Bronchial Epithelial Cells Grown from Explants
14:32

Primary Human Bronchial Epithelial Cells Grown from Explants

Published on: March 26, 2010

Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells
10:46

Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

Published on: June 14, 2022

Main Results:

  • A robust method for growing primary human bronchial epithelial cells from explants is established.
  • The technique yields an abundant supply of cells suitable for various research purposes.
  • Cultured cells can be successfully expanded and differentiated.

Conclusions:

  • The described method provides a reliable source of primary human bronchial epithelial cells.
  • These cells serve as valuable models for studying respiratory diseases and for drug screening.
  • This approach supports advancements in respiratory medicine and toxicology research.