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Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
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Desorption corona beam ionization source for mass spectrometry.

Hua Wang1, Wenjian Sun, Junsheng Zhang

  • 1Shimadzu Research Laboratory (Shanghai) Co., Ltd., Shanghai, 201201, P. R. China.

The Analyst
|March 31, 2010
PubMed
Summary

A new Desorption Corona Beam Ionization (DCBI) source enables direct surface analysis in mass spectrometry without sample pretreatment. This novel technique facilitates localized sampling and component separation in complex mixtures.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Direct analysis of samples from surfaces in mass spectrometry often requires extensive sample preparation.
  • Existing ambient ionization techniques, like Direct Analysis in Real Time (DART), have limitations in spatial resolution and sample separation.

Purpose of the Study:

  • To introduce a novel Desorption Corona Beam Ionization (DCBI) source for direct surface analysis.
  • To demonstrate the capabilities of DCBI for localized sampling, imaging, and profiling.
  • To evaluate DCBI's potential for analyzing complex mixtures through temperature-controlled desorption.

Main Methods:

  • Development of a DCBI source utilizing a hollow needle/ring electrode structure for corona discharge.
  • Operation under ambient conditions without sample pretreatment.
  • Implementation of progressive temperature scans from room temperature to 450°C for sequential desorption.

Main Results:

  • The DCBI source generates a visible corona beam, enabling precise localization of sampling areas.
  • Progressive temperature scans allow for sequential desorption and rough separation of components in complex mixtures, reducing spectral congestion.
  • Successful acquisition of mass spectra for diverse compounds including pesticides, veterinary additives, drugs, and explosives.

Conclusions:

  • The DCBI source offers a versatile platform for direct surface analysis in mass spectrometry.
  • Its ability to perform localized sampling and temperature-programmed desorption enhances its utility for complex sample analysis.
  • DCBI provides a valuable alternative to existing ambient ionization methods, particularly for spatially resolved and component-separated analyses.