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Updated: Jun 14, 2026
Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation
Published on: September 16, 2018
[Study on IgG promoter as probe to evaluate safety of injections in pre-clinic]
Yugang Wang1, Lele Li, Fan Lei
1Laboratory of Pharmaceutical Science, Department of Biological Sciences and Biotechnology, School of Medicine, Tsignghua University, Beijing 100084, China.
Objective:
To construct an effective system screening and evaluating possible injections and components inducing allergy type II.
Method:
Transfect IgG promoter-regulated green fluorescent protein expressing plasmid into RPMI-8226 cell. The number of fluorescent cells after drug treatment was calculated and statistically evaluated.
Result:
Tween 80 can suppress the expression of IgG effectively, and puerarin has activity of stimulating IgG expression, and the system has no response to KCl-treatment. No effect of Yuxingcao (Herba Houttuyniae) injection on this system is observed.
Conclusion:
IgG promoter-drove green fluorescent protein expressing cell line can be used as system screening injections and components inducing allergy type II based on stimulating IgG promoter activity. Tween 80 can suppress the expression of IgG at the transcriptional level. Four batches of Yuxingcao injection cannot induce allergy type II by activating IgG expression.
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