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Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

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Published on: February 1, 2022

Biocompatible graphene oxide-based glucose biosensors.

Yong Liu¹, Dingshan Yu, Chao Zeng

¹Biomedical Engineering Academy, School of Ophthalmology & Optometry, Wenzhou Medical College, 270 Xueyuan Road, Wenzhou 325027, China. yongliu1980@hotmail.com

Langmuir : the ACS Journal of Surfaces and Colloids

|March 31, 2010

Summary

A novel graphene oxide-based biosensor for glucose detection shows high efficiency and stability. This enzyme electrode also demonstrates excellent biocompatibility with human cells, paving the way for clinical diagnostic applications.

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NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose

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Area of Science:

Biomaterials Science
Biosensor Technology
Nanotechnology

Background:

Development of efficient biosensors is crucial for disease diagnosis.
Graphene oxide (GO) offers unique properties for biosensor development.
Immobilization of enzymes on nanomaterials enhances biosensor performance.

Purpose of the Study:

To develop a highly efficient enzyme electrode using covalent attachment of glucose oxidase (GOx) to graphene oxide (GO).
To investigate the electrochemical performance and stability of the GOx-GO biosensor.
To evaluate the biocompatibility of GO nanosheets with human retinal pigment epithelium (RPE) cells.

Main Methods:

Covalent attachment of glucose oxidase (GOx) to carboxyl groups of graphene oxide (GO) sheets.
Electrochemical characterization of the resulting enzyme electrode.
Assessment of biosensor sensitivity, linear range, reproducibility, and storage stability.
In vitro biocompatibility testing of GO nanosheets with human RPE cells.

Main Results:

A novel, highly efficient enzyme electrode was successfully fabricated via GOx-GO covalent linkage.
The biosensor demonstrated a broad linear range (up to 28 mM) and high sensitivity (8.045 mA x cm(-2) x M(-1)).
The GOx-GO electrode exhibited excellent reproducibility, storage stability, and good biocompatibility with RPE cells.

Conclusions:

The developed GOx-GO enzyme electrode is a promising platform for sensitive and stable glucose detection.
Graphene oxide nanosheets show good biocompatibility, supporting cell attachment and proliferation.
This research highlights the potential of graphene-based biosensors for clinical diagnosis and biomedical applications.