Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Occurrence and potential sources of greenhouse gases at Wuzhen, a typical ancient water town in Eastern China.

Environmental monitoring and assessment·2026
Same author

Incipient Constituents: Phonesthemes Facilitate Word Processing in English.

Open mind : discoveries in cognitive science·2026
Same author

Self-Assembed G-Quadruplex Nanowires for Energy Transfer over Micrometers.

Biomacromolecules·2026
Same author

Soil legacy effects of long-term nitrogen addition on litter decomposition and soil CO<sub>2</sub> efflux in a subtropical grassland: a short-term incubation study.

Frontiers in plant science·2026
Same author

[A two-stage model for predicting postoperative pulmonary infection in esophageal cancer patients].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi·2026
Same author

Dual-Function Electrocatalytic Activity Unleashed by FeMo-Graphdiyne@Ni<sub>3</sub>S<sub>2</sub> with Engineered Heterointerfaces.

Precision chemistry·2026

Related Experiment Video

Updated: Jun 14, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Hand-held photometer based on liquid-core waveguide absorption detection for nanoliter-scale samples.

Jian-Zhang Pan1, Bo Yao, Qun Fang

  • 1Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.

Analytical Chemistry
|March 31, 2010
PubMed
Summary
This summary is machine-generated.

A novel hand-held photometer uses liquid-core waveguide technology for analyzing nanoliter samples. This compact device enables on-site quality and quantity assessment of DNA and cholesterol, improving portability and reducing sample volume.

More Related Videos

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
05:16

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

Published on: October 25, 2021

Related Experiment Videos

Last Updated: Jun 14, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
05:16

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

Published on: October 25, 2021

Area of Science:

  • Analytical Chemistry
  • Biophotonics
  • Instrumentation

Background:

  • Traditional photometers often require larger sample volumes and are not easily portable.
  • Nanoliter-scale sample analysis is crucial for efficient and cost-effective biochemical assays.
  • Integrating multiple optical and electronic components into a compact device presents engineering challenges.

Purpose of the Study:

  • To develop a fully integrated, hand-held photometer utilizing liquid-core waveguide (LCW) technology.
  • To enable on-site analysis of nanoliter-scale samples for biochemical applications.
  • To demonstrate the device's utility in assessing DNA and serum cholesterol levels.

Main Methods:

  • Integration of all photometer components (LED light source, LCW flow cell, photodiode detector, pump, electronics, display, battery) into a compact instrument (12 x 4.5 x 2.1 cm).
  • Development of a bent optical coupler for efficient light coupling into/out of the LCW flow cell via its sidewall.
  • Utilized dual UV-LEDs (260 nm and 280 nm) for dual-wavelength detection within a Teflon AF capillary integrating sampling and optics.

Main Results:

  • Achieved a fully integrated, hand-held photometer with a sample consumption of only 350 nL and an effective optical path length of approximately 15 mm.
  • Demonstrated successful on-site assessment of DNA quality and quantity using dual-wavelength detection.
  • Validated the photometer's potential for point-of-care testing through serum total cholesterol measurements.

Conclusions:

  • The developed hand-held photometer offers a simplified, reliable, and low-sample-volume solution for on-site biochemical analysis.
  • The LCW detection principle and integrated design are suitable for portable analytical instrumentation.
  • The device shows promise for applications in nucleic acid quantification and point-of-care diagnostics.