Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of prophylactic subcutaneous unfractionated heparin on the maternal sFlt-1/PlGF ratio: a retrospective cohort study.

BMC pregnancy and childbirth·2026
Same author

[Spontaneous resolution of ATRA-related hearing impairment without symptoms of intracranial hypertension].

[Rinsho ketsueki] The Japanese journal of clinical hematology·2026
Same author

Accelerated Discovery-to-Unveiling of High-Performance and Affordable Ammonia Electrode Process by Human-Machine Collaboration Framework.

Angewandte Chemie (International ed. in English)·2026
Same author

Mother-child correlations in hair mineral profiles in Japan: Insights from a cross-sectional study.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)·2026
Same author

Atomic-scale elucidation of formation and structure in high-performance Re-Ge nanocatalysts.

Nanoscale·2026
Same author

Experimental demonstration of optical cloaking of a free-standing Ag nanowire.

Optics letters·2026

Related Experiment Video

Updated: Jun 14, 2026

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell
08:00

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

Published on: July 19, 2015

Three-dimensional optical sectioning by scanning confocal electron microscopy with a stage-scanning system.

Ayako Hashimoto1, Masayuki Shimojo, Kazutaka Mitsuishi

  • 1International Center for Young Scientists, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba 305-0047, Japan. Hashimoto.Ayako@nims.go.jp

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|March 31, 2010
PubMed
Summary

Annular dark-field scanning confocal electron microscopy (ADF-SCEM) with stage-scanning offers improved depth resolution for 3D imaging. This technique effectively visualizes nanoparticle depth and position on nanostructures.

More Related Videos

Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping
09:40

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping

Published on: February 20, 2026

Related Experiment Videos

Last Updated: Jun 14, 2026

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell
08:00

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

Published on: July 19, 2015

Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping
09:40

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping

Published on: February 20, 2026

Area of Science:

  • Electron Microscopy
  • Nanotechnology
  • Materials Science

Background:

  • Three-dimensional (3D) imaging is crucial for understanding nanoscale structures.
  • Existing techniques like scanning transmission electron microscopy (STEM) and bright-field SCEM have limitations in depth resolution.
  • Annular dark-field scanning confocal electron microscopy (ADF-SCEM) is a novel 3D imaging technique.

Purpose of the Study:

  • To evaluate the depth resolution of ADF-SCEM utilizing a stage-scanning system.
  • To demonstrate the capability of ADF-SCEM for depth sectioning of nanoparticles.
  • To apply ADF-SCEM for analyzing the 3D positioning of catalytic nanoparticles.

Main Methods:

  • Utilized an ADF-SCEM instrument with a pinhole aperture before the detector and an annular aperture under the specimen.
  • Employed a stage-scanning system to acquire slice images perpendicular and parallel to the optical axis.
  • Observed nanoparticles to assess depth resolution and elongation along the optical axis.

Main Results:

  • ADF-SCEM demonstrated effective depth sectioning by significantly reducing nanoparticle elongation compared to STEM and bright-field SCEM.
  • Experimentally determined nanoparticle length closely matched theoretical estimations based on probe size.
  • Successfully applied ADF-SCEM to analyze the 3D positions of catalytic nanoparticles on carbon nanostructures.

Conclusions:

  • ADF-SCEM with stage-scanning provides superior depth resolution for 3D imaging of nanoscale materials.
  • The technique accurately visualizes nanoparticle dimensions and spatial distribution.
  • ADF-SCEM is a promising tool for advanced nanoscale analysis, particularly for catalytic nanoparticles.